Researchers at École polytechnique fédérale de Lausanne (EPFL), Lausanne, Switzerland, have discovered that bile acids curb appetite by entering the brain. Their findings featured in the paper, ‘Central anorexigenic actions of bile acids are mediated by TGR5’, published in Nature Metabolism, provide new insights into the signals and mechanisms by which satiety is controlled and may have implications for treating obesity.
Our brain is usually well protected from uncontrolled influx of molecules from the periphery thanks to the blood-brain barrier, a physical seal of cells lining the blood vessel walls. The hypothalamus, however, is a notable exception to this rule. Characterised by ‘leaky’ blood vessels, this region, located at the base of the brain, is exposed to a variety of circulating bioactive molecules. This anatomical feature also determines its function as a rheostat involved in the coordination of energy sensing and feeding behaviour.
Several hormones and nutrients are known to influence the feeding neurocircuit in the hypothalamus. Classic examples are leptin and insulin, both involved in informing the brain of available energy. In the last years, the list of appetite or satiety triggering signals has been steadily growing with the identification of several gut hormones. Those are involved in fine-tuning feeding behaviour by regulating the perception of hunger or satiety, ultimately leading to the initiation or termination of a meal. The gut-brain axis is thus a critical gatekeeper in regulating feeding behaviour.
Bile acids are among the most abundant metabolites in the gut and act as versatile signalling molecules that relay nutrient availability to a physiological response by activating the bile acid responsive membrane receptor, Takeda G-coupled receptor 5 (TGR5). Although the ancient Greeks already postulated that bile may affect our state of mind, we know very little about the signalling role of these metabolites in the brain.
In the study, EPFL together with the EPFL Brain Mind Institute and Bertarelli Platform for Gene Therapy, and several collaborators from France, Italy and the US, the authors showed that bile acids reach the mouse brain shortly after a meal to suppress food intake. Bile acids escape the digestive tract, transiently accumulate in the blood circulation, and spike in the hypothalamus for a very short period of time after feeding. The authors demonstrated that the anorexic response of bile acids is mediated by TGR5, located at the cell surface of a distinct group of hypothalamic cells, called AgRP/NPY neurons. When focusing on this neuronal subpopulation, they found that bile acids mediate two processes staggered in time.
"While bile acids acutely block the release of appetite-stimulating AgRP and NPY peptides during the first minutes following binding of their cognate receptor, they further reinforce the repression by blunting the expression of these neurotransmitters," said Dr Alessia Perino, first-author of the paper.
Over the last two decades, bile acids have been proven to be efficacious in alleviating chronic metabolic and inflammatory disorders. Previous studies have demonstrated that systemic TGR5 activation attenuates obesity in diet-induced obese mice. The current study reveals that the bile acid-TGR5 signalling axis is not only important in disease, but also in the physiological control of eating behaviour. In the absence of dietary fat, bile acids temporarily suppress food intake without affecting the normal energy balance.
"This is not surprising as homeostasis is about a self-regulatory process in which systems tend to maintain stability" added Associate Professor, Kristina Schoonjans. "In contrast, chronic high fat diet feeding may override this equilibrium. It will be interesting to find out whether the identified neurocircuits contribute to the known body weight reducing effect of bile acids in the setting of diet-induced obesity."