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Researchers design strategy to fight obesity through gene therapy

Researchers from the University of Barcelona and CIBERobn have designed a strategy to fight obesity and diabetes in mice through ex vivo gene therapy which consists of implanting cells that have been manipulated and transformed in order to treat a disease. The research team believes this is the first study to apply the ex vivo gene therapy technique to generate and implant cells that express the CPT1AM protein, an enzyme that plays a decisive role in many metabolic diseases such as obesity.

Graphical abstract. Credit: Metabolic Engineering (2023). DOI: 10.1016/j.ymben.2023.04.010

"In this new therapy, animal models have been implanted subcutaneously stem cells derived from adipose tissue, differentiated into adipocytes, so that they can express an active form of the CPT1AM protein, an enzyme located in the mitochondria that is key in lipid oxidation and is related to metabolic diseases," said Professor Laura Herrero, from the Faculty of Pharmacy and Food Sciences and the Institute of Biomedicine of the University of Barcelona (IBUB), and from the Physiopathology of Obesity and Nutrition Networking Biomedical Research Centre (CIBERobn). "As a result, in obese mice, it has been possible to reduce weight, hepatic steatosis, cholesterol and glucose levels. In conclusion, the implantation of adipocytes expressing the mitochondrial enzyme CPT1AM helps to reduce obesity and glucose intolerance in mice."

Cell therapy describes the process of introducing new cells into a tissue in order to fight a disease. Cell therapies are currently focused on addressing hereditary diseases - with and without the help of gene therapy - or degenerative diseases. As the cell transformation process takes place outside the body of the organism, this type of therapy is much easier to carry out and allows for greater control of the altered cells.

"Specifically, carnitine palmitoyltransferase 1A (CPT1A) is the enzyme that controls mitochondrial fatty acid oxidation. Our aim was to generate adipocytes that could express a constitutively active form of CPT1A - CPT1AM - capable of burning excess fat and improving the obese metabolic phenotype of mice after implantation."

The results of the study support the future clinical use of this ex vivo gene therapy approach as a new strategy to reduce obesity and cholesterol rates in the population. This preclinical study could open the doors to future therapeutic strategies to address the treatment of obesity, which today represents a global health problem.

"To approximate the therapy in humans, we need to optimize several processes such as the quality and viability of stem cells from adipose tissue isolated from people with obesity, the percentage of infection with lentivirus, and the number of cells used for transplantation," concluded Herrero.

The findings were featured in the paper, ‘Implantation of CPT1AM-expressing adipocytes reduces obesity and glucose intolerance in mice’, published in Metabolic Engineering.

To access this paper, please click here


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